Fig. 4.
mHtt expression is associated with increased ER stressor-induced cell death in striatal cells expressing full-length Htt. (A) STHdhQ7/7 and STHdhQ111/111 cells untreated (control) or treated with 5 μg/ml Tm for 16 hours. Cells were fixed and labeled with cleaved caspase-3 antibody and phalloidin. Scale bars: 20 μm. (B) The percentage of cells with cleaved caspase-3 staining. *P<0.01. (C) STHdhQ7/7 and STHdhQ111/111 cells treated with 1 (1.0 Tm) and 5 μg/ml Tm (5.0 Tm) for 16 hours. STHdhQ7/7 cells were also treated with 5 μM staurosporine for 5 hours as positive control (S). Cells were processed for immunoblotting for cleaved caspase-3. Immunoblots were then reprobed with anti-α-tubulin as a loading control.