Fig. 7.
Adaptation to ER stress in striatal cells expressing full-length Htt. (A) Representative fluorescence images of STHdhQ7/7 and STHdhQ111/111 cells either left untreated or treated with 5 mM DTT for 30 minutes and followed by 16 hours washout of the drug. Cells were fixed and immunofluorescently stained with anti-BiP. Scale bar: 20 μm. (B) D values of single STHdhQ7/7 and STHdhQ111/111 cells transfected with BiP–GFP for 16 hours, and either left untreated (Naive) or treated with 5 mM DTT for 30 minutes (Adapted) and followed by a 16-hour washout of the drug. Naive or Adapted cells were subsequently challenged with 5 μg/ml Tm for 4 hours (+Tm), and analyzed by FRAP. (C) STHdhQ7/7 and STHdhQ111/111 cells either left untreated (Naive) or treated with 5 mM DTT for 30 minutes (Adapted) and followed by a 16-hour washout of the drug. Naive or Adapted cells were subsequently challenged with 5 μg/ml Tm for 16 hours, fixed and labeled with anti-cleaved caspase-3 and phalloidin. The percentage of cells with cleaved caspase staining was then determined. n>82 cells per data set. *P<0.01, **P<0.0005.