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Phenotypic markers for Hp deficiency in C57BL/6J mice. Comparison of age-matched (8-month-old) Hp+/+ and Hp−/− mice: (A) black and shining hair; (B) smaller spleen size; (C) lower number and smaller size germinal centers in spleen (H&E staining); and (D) presence of Hb precipitates within splenocytes (bottom view of the pellet of isolated cells in centrifuge tube).
