Figure 2.

LPS-mediated induction of acute phase reactants in various organs is maintained in Hp^−/− mice. (A) Age-matched (10-week-old) Hp^+/+ and Hp^−/− C57BL/6J mice received a single i.p. injection of PBS (Ctrl) or 50 μg LPS, and after 24 h (Ctrl and LPS) or 96 h (LPS), RNAs were extracted from the indicated organs of two animals and analyzed by Northern blotting for the mRNA listed on the right. (B) A separate set of 10-week-old animals received PBS (Ctrl) or 50 μg LPS, and 24 h later, liver RNAs were isolated and analyzed by Northern blotting for the indicated mRNAs. SAA, Serum amyloid A; EtBr, ethidium bromide; TIMP-1, tissue inhibitor for metalloproteinase-1; HO, heme oxygenase.