Figure 8.

Attenuated response of CD8+ T cells from Hp^−/− mice. (A) CD8⁺ OT-1 cells were isolated from spleens of Hp^+/+ or Hp^−/− C57BL/6J background and cultured in the presence of irradiated BOK cells presenting H-2K^b-SIINFEKL and B7.1. DNA synthesis ([³H]Thymidine Incorporation) at Days 2–5 was determined. (B) The relative presence of apoptotic cells in the cultures at the times indicated was determined by flow cytometric analysis using labeling with Annexin V-FITC and PI. (C) CFSE-labeled, naive OT-1 T cells from Hp^+/+ or Hp^−/− at the onset and after 96 h stimulation by coculture with BOK cells were analyzed by flow cytometry. (D) OT-1 T cells from Hp^+/+ or Hp^−/− were stimulated by coculture with BOK cells for 72 h, and conditioned medium was analyzed for concentration of IFN-γ and IL-2.