Figure 6.

SGE induces down-regulation of DC surface molecule expression. DC cells previously incubated overnight with SGE from P. papatasi or PBS were cultured in the presence of LPS during 24 h. Surface molecules were labeled with FITC or PE conjugated with anti-MHC class-II, anti-CD80, anti-CD86, or anti-CD40 antibodies. (A) The representative geometric mean fluorescence intensity (MFI) of DC staining from LPS or LPS + SGE cultures is shown in each box. The filled histograms represent cells labeled with the specific mAb; the empty histograms represent the same cell suspension labeled with isotypic control mAb. (B) The bars display the relative MFI obtained from one of four independent experiments made in duplicate (n=2); *, P < 0.05, compared with the LPS stimulation.