TABLE 1.
Effect of Sand Fly SGE on Leukocyte Migration Induced by OVA in Immunized Mice
| Cells | False-immunized |
OVA-immunized |
|||
|---|---|---|---|---|---|
| PBS (i.v.) | PBS (i.v.) | OVA (i.p.) | |||
| OVA (i.p.) | PBS (i.p.) | PBS (i.v.) | P. papatasil (i.v.) | P. duboscqi (i.v.) | |
| Total leukocytes | 4.33 × 106 ± 1.2 | 5.25 × 106 ± 3.6 | 12.33 × 106 ± 3.1a | 9.42 × 106 ± 3.7b | 8.50 × 106 ± 3.8b |
| Neutrophils | 12.0 ± 0.14 (29%) | 0.06 ± 0.0003 (1.1%) | 71 ± 0.31 (58%)a | 0.65 ± 0.015 (6.6%)b | 0.8 ± 0.20 (9.3%)b |
| Eosinophils | 2.0 ± 0.01 (4.6%) | 3.0 ± 0.02 (5.7%) | 20 ± 0.1 (16.2%)a | 57.1 ± 7.0 (60.3%)b | 43.0 ± 6.0 (50.3%)b |
| Macrophages | 1.0 ± 0.1 (2.1%) | 0.4 ± 0.1 (0.7%) | 3.2 ± 0.8 (2.6%)a | 0.32 ± 0.6 (0.33%)b | 0.6 ± 0.04 (0.72%)b |
| B cells | 0.25 ± 0.02 (0.6%) | 0.23 ± 0.04 (0.2%) | 2.1 ± 0.1 (1.7%)a | 0.2 ± 0.06 (0.2%)b | 0.5 ± 0.2 (0.7%)b |
| CD4+T | 0.4 ± 0.2 (0.9%) | 0.2 ± 0.09 (0.36%) | 1.2 ± 0.3 (0.9%)a | 0.2 ± 0.04 (0.2%)b | 0.4 ± 0.08 (0.5%)b |
| CD8+T | 0.002 ± 0.0004 (0.004%) | 0.003 ± 0.001 (0.006%) | 0.014 ± 0.004a (0.01%) | 0.0052 ± 0.002b (0.005%) | 0.0023 ± 0.001b (0.003%) |
The data are mean ± sd of the number of leukocytes recovered from the peritoneal cavity of OVA-immunized and control (sham-immunized) mice treated with PBS or SGE from P. papatasi or P. duboscqi vector (one gland/i.v./animal) 48 h before i.p. challenge with PBS or OVA (10 μg). The values inside parenthesis mean the percentage of each cellular type relative to the total leukocyte number. The values are ×105 per peritoneal cavities. Experimental groups included four to five animals.
a
P < 0.05 compared with control groups;
b
P < 0.05 compared with the PBS-pretreated OVA-immunized group and challenge with OVA (PBS + OVA).