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. 2008 Apr 28;84(1):115–123. doi: 10.1189/jlb.1207835

Figure 1.

Figure 1

Binding of PBMC by anti-SP-R210 antibodies. After blocking in FACS staining buffer, PBMC were incubated with α-SP-R210ct for 30 min on ice. The cells were washed and further incubated with FITC-conjugated mouse anti-rabbit secondary antibody in combination with PE-CD14 or PE-CD3 antibodies for an additional 30 min. The cells were then washed and analyzed by flow cytometry. (A) Representative dot plots of freshly isolated PBMC, stained with anti-SP-R210 and anti-CD14 or anti-CD3 as indicated. The percentages of double-positive cells are shown in the upper right quadrant of each dot plot. (B) The mean ± se of cell populations and cells that express SP-R210 from four different donors. (C) Representative dot plots of SP-R210+ and CD3+ lymphocytes in PBMC, cultured in the absence (left panel) or presence (right panel) of heat-killed M. tuberculosis. (D) The mean ± se of SP-R210-expressing CD3+ cells from four donors, cultured with medium alone or with heat-killed M. tuberculosis.