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. 2008 Apr 28;84(1):115–123. doi: 10.1189/jlb.1207835

Figure 2.

Figure 2

Elevated expression of SP-R210 by PBMC in response to M. tuberculosis stimulation. Total cell protein extracts (35 μg) of PBMC from a PPD+ donor cultured in medium alone for 48 h or stimulated with heat-killed M. tuberculosis for 24 and 48 h were resolved in 7.5% SDS-PAGE, followed by electroblotting to a nitrocellulose membrane, which was then blocked and blotted for expression of SP-R210 with α-SP-R210ct (upper panel). The membrane was subsequently reblotted for β-actin (lower panel) as a protein loading control for each sample after stripping. One representative result from three different experiments is shown.