Figure 6.

Neutralization of IL-10 and TGF-β1 abrogates the inhibitory effect of α-SP-R210n on M. tuberculosis-induced production of IFN-γ. PBMC were cultured in medium alone or with 2.5 μg/ml heat-killed M. tuberculosis in the absence or presence of different IgG (10 μg/ml) as indicated. In some cases, 1 μg/ml neutralizing antibodies to IL-10 and TGF-β1 were also added. After 48 h incubation, IFN-γ levels in the culture supernatants were measured by ELISA (A), cells were collected, and IFN-γ mRNA expression was measured by real-time PCR, normalized to 18S rRNA content (B). The mean ± se of five (A) or six (B) donors is shown.