Table 1.
Primers used in this study
| name | Sequence | Coordinates according to Genbank |
|---|---|---|
| Bo1 Fwd | 5'- ATGGAGGGTGATGGATTCATG-3' | 460-440a |
| Bo1 Rev | 5'- TTAAGGCCTCATTCCAGGAAG-3' | 272-292a |
| Bo5 Fwd | 5'- GCTACAGAAAATGGCCAGTAAAG-3' | 20366-20342a |
| Bo5 Rev | 5'- TCATGTCCTGAGTGGGTCTATG-3' | 19170-19191a |
| Bo6 Fwd | 5'- ATGGTCATCCTAAATGCTCAAG -3' | 20297-20318a |
| Bo6 Rev | 5'- TCACCTAGTGTTGCAACCCC -3' | 20497-20478a |
| Bo7 Fwd | 5'- ATGGAGACAATTTCCATAAACTG -3' | 20994-20972a |
| Bo7 Rev | 5'- CTAGCTGGGGTAGAGTGATC -3' | 20671-20690a |
| ORF67.5 Fwd | 5'- ATGGCTGATGGTGATGTTTTAG -3' | 93144-93123a |
| ORF67.5 Rev | 5'- TCAATGTTTGTCCAGAGCACT -3' | 92881-92901a |
| Bo12 Fwd | 5'- ATGGGGGCGCTATTTGGGC -3' | 97442-97460a |
| Bo12 Rev | 5'- TCAACTGATGAAACCCACCC -3' | 97525-97506a |
| Bo13 Fwd | 5'- ATGCGTCTCGATGGCAAGC -3' | 98838-98856a |
| Bo13 Rev | 5'- CTATGGTTGTTTTTTAAAGAAAATC -3' | 98981-98957a |
| ORF75 Fwd | 5'- ATGTATCCCAGATACAGTAACA -3' | 103606-103585a |
| ORF75 Rev | 5'- TTACATTTTATTTTTCAGACACCA -3' | 100274-100297a |
| prDNA Fwd 1 | 5'- GGAGCCCAAAACCAAAAGAG -3' | 870-889b |
| prDNA Rev 1 | 5'- CTCTTTTGGTTTTGGGCTCC -3' | 889-870b |
| prDNA Fwd 2 | 5'- CGTAGGCCTCACATTCAGC -3' | 908-926b |
| prDNA Rev 2 | 5'- GCTGAATGTGAGGCCTACG -3' | 926-908b |
| prDNA Fwd 3 | 5'- CGAGAGATGGTTCTTGCACA -3' | 940-959b |
| prDNA Rev 3 | 5'- TGTGCAAGAACCATCTCTCG -3' | 959-940b |
| BAC Rev | 5'- TTGCCAATCCCAAAAAGAAG -3' | 9859-9878c |
a according to Genbank JN133502 sequence (BoHV-4 V.test strain long unique region)
b according to Genbank JN133504 sequence (BoHV-4 V.test strain prDNA inner region)
c according to Genbank AY665170.1 sequence (pBeloBAC modified)