Figure 7. UPS targets proteins involved in translational silencing and synaptic structure in the amygdala following memory retrieval.

[A] Experimental design for B-D. Animals were trained to auditory or context fear conditioning on Day 1. The next day, animals were exposed to a brief retrieval and amygdala tissue collected 60-min [context, n = 10] or 90-min [auditory, n = 10] later. Two separate groups received auditory or context fear conditioning on Day 1 and were sacrificed on Day 2 without receiving retrieval [n = 6 per group]. Amygdala tissue was fractionated to obtain a crude synaptosomal membrane fraction, purified with GST-S5a, and probed with antibodies against MOV10, Shank and NR2B [B]. The amount of polyubiquitinated synaptic MOV10 [C] and Shank [D], was increased following fear memory retrieval. * denotes p<.05 from No React controls.