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. 2011 Sep 22;6(9):e24508. doi: 10.1371/journal.pone.0024508

Figure 6. RCAN3 isoform predicted protein sequences aligned by ClustalW software.

Figure 6

RCAN3-2,3,4,5 here refers to RCAN3-1,2,3,4,5, RCAN3-1a,2,3,4,5, RCAN3-1b,2,3,4,5 and RCAN3-1c,2,3,4,5 mRNA products. RCAN3-2,4,5 refers with certainty to RCAN3-1c,2,4,5 mRNA product. In all these isoforms the coding sequence starts in exon 2 and finishes in exon 5. RCAN3-4,5 refers to RCAN3-1,3,4,5, RCAN3-1a,3,4,5 and RCAN3-1c,3,4,5 product sequences. In all these isoforms the coding sequence starts in exon 4. Due to the lack of exon 2, RCAN3-4,5 sequence is shorter than RCAN3-1,2,3,4,5 protein and lacking the amino terminus containing the exon 2 product necessary for human cardiac troponin I (TNNI3) binding. Due to out-of-frame joining between exons 2 and 5 and between exons 3 and 5, the carboxyl terminus sequence encoded by RCAN3-2,5 and by RCAN3-2,3,5 cDNAs, respectively, is not similar to that of RCAN3-1,2,3,4,5 product sequence. In bold and italic the initial methionine of the proteins. Black border box: exon 2 encoded amino acids; shaded dark grey residues: FLISPP motif. Shaded light grey: CIC motif, with the embedded ELHA motif (KYELHAGTESTPS). The diagram shows the longest protein sequences available to date (see mRNA GenBank accession numbers in text or figure 1). Alignment was made by ClustalW software and manual adjustment.