Table 1. Primer list.
| No | Primer sequence 5′ → 3′ | gene or plasmid c | specificity d |
| #1 | CTCCCGTCCTGCGGCGGCGGGGCGT (F)a | RCAN3-1 | exon 1/exon 1c |
| #2 | CAGAGTCTCACCTATGCTGTTCG (R)b | RCAN3 isoforms | exon 5 |
| #3 | GTGCTAGCTCCAGCTCAGCTACCT (F) | RCAN3-1a | exon 1a |
| #4 | AGATAGGACTTGTCCCGCACTTCGC (R) (R) | RCAN3-1a * | exon 4 |
| #5 | GTATAACAGAAAAATGAACCAAGCACT (F) | RCAN3-1b | exon 1b |
| #6 | CCATCATCTCATCCAAATCATCTTC (R) | RCAN3-1b * | exon 2 |
| #7 | GCGGCCGCAGGTAGCTGAGCTG (F) | RCAN3AS | exon 1 |
| #8 | ATGCTTTGTAGAAGTACTACAGGGA (R) | RCAN3AS | exon 3 |
| #9 | TGGGGCGGCAGGAGATAGGACTGTG (R) | RCAN3-2,3,4b,5 | exon 4b-exon 3 |
| #10 | TCCCGCGTGAAGTTCATATTTCTCTCCTTC (R) | RCAN3-2,5 | exon 5-exon 2 |
| #11 | CACTTCGCCGGACATCTGCACCTTC (R) | RCAN3-2,4,5 | exon 4-exon 2 |
| #12 | CGTGAAGTTCATATTTCTCTCCTGTGCAA (R) | RCAN3-2,3,5 | exon 5-exon 3 |
| #13 | AGGGTTTTCCCAGTCACGAC (F) | pcR 2.1 plasmid | |
| #14 | CAGGAAACAGCTATGACCATGA (R) | pcR 2.1 plasmid | |
| #15 | CGCCCGGCCTCTCCAGCAGGAAAG (F) | RCAN3-1,3,4,5 | exon 1-exon 3 |
| #16 | CGGCGGCGGGGCGTGCAGGAAAG (F) | RCAN3-1c,3,4,5 | exon 1c-exon 3 |
| #17 | TTGCCGGGAGGTGAACCGAGGAAAG (F) | RCAN3-1a,3,4,5 | exon 1a-exon 3 |
| #18 | GATTTCTGGCACGATAAATGAGAAAG (F) | RCAN3-1b,3,4,5 | exon 1b-exon 3 |
| #19 | GCTGATGGCGATGAATGAACACTG (F) | 5′ Race Outer | |
| #20 | TCACAGACATGAACCACCACGCTG (R) | RCAN3 isoforms | exon 5 |
| #21 | CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG (F) | 5′ Race Inner | |
| #22 | TGTCGACTCTGTTCCCGCGTGAAG (R) | RCAN3 isoforms | exon 5 |
| #23 | CTTCGCCGGACATCTGCACCTGTGCAA (R) | RCAN3-1b * | exon 4-exon 3 |
| #24 | TGCGGCGGCGGGGCGTGCAGGTGAG (F) | RCAN3-1 | exon 1 |
| #25 | GTCCTGCGGCGGCGGGGCGTGCAGTGG (F) | RCAN3-1c | exon 1c-exon 2 |
| #26 | GCTACACAGATCTGACTGGCTATCATTC (R) | RCAN3-1/1c * | exon 2 |
a
F, forward primer.
b
R, reverse primer.
c
RCAN3-1: isoforms with exon 1; RCAN3-1a: isoforms with exon 1a; RCAN3-1b: isoforms with exon 1b; RCAN3AS: all RCAN3AS isoforms;
*: star indicates reverse primers used for specific alternative first exon isoforms cloning, but useful to clone all isoforms containing exon 2 or exon 3 or exon 4.
d
When two exon numbers are provided, the primer encompasses the boundary between the two exons.