Figure 1. Heterotrimeric VSV N_Δ21 ⁰-P dimer complex in solution.

The complex formed between N_Δ21 and full-length P dimer in solution was analyzed by SEC-MALLS. The N_Δ21 ⁰-P complex elutes at 12.0 mL and the remaining N_Δ21 ⁰-P₆₀ complex elutes at 15.6 mL (line). The molecular mass of 104±4 kDa (crosses) indicates a 1∶2 complex between one RNA-free N_Δ21 and two P molecules in accordance with the previous observation that P forms exclusively dimers in solution and with the N⁰-P₂ complex determined for rabies virus.