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. 2011 Sep 22;7(9):e1002255. doi: 10.1371/journal.ppat.1002255

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Ning et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Native HBV NCs purified by sucrose gradient centrifugation from transfected HepG-2 cells (WT, bottom left; Pol^-, bottom right) were visualized by EM after negative staining with phosphotungstic acid. E. Coli-derived HBV WT (top left) or C-terminally truncated (at position 144, C144 or ΔCTD; top right) capsids were examined in parallel as (RNA) filled and empty capsid controls, respectively. Two WT capsids with distinct appearances, empty (arrowhead) and filled (arrow), are indicated.