Figure 2. FTIR analysis of the dried film obtained after spin drying of sample buffer (1.8 M trehalose, 10 mM KCl, 5 mM glucose, 20 mM HEPES, 120 mM choline chloride, pH 7.4).

The ratio of I₁₆₅₀/I₁₁₅₀ was used to quantify local water contents.