Skip to main content
. 2011 Sep 22;7(9):e1002263. doi: 10.1371/journal.ppat.1002263

Figure 3. Isolation of M. pneumoniae glycerophosphodiesterases transposon insertion mutants.

Figure 3

(A, C) Schematic representation of the genomic region surrounding the glpQ and mpn566 gene (both designated as glycerophosphodiesterases) in M. pneumoniae and site of the transposon insertion in the knockout strains GPM81 and GPM82, respectively. The annealing sites of oligonucleotides used for the determination of the transposon insertion site are indicated by small arrows. Probes A and B hybridizing to internal fragments of the glycerophosphodiesterases and the aac-ahpD genes are depicted as dotted lines. (B, D) Southern blot analysis to confirm the single insertion of the mini-transposon into the glpQ and mpn566 gene of the strains GPM81 and GPM82, respectively. Chromosomal DNAs of the wild type and both glycerophosphodiesterase mutants were digested as indicated. Blots were hybridized with the respective glycerophosphodiesterase-specific probe (left) and a probe hybridizing to the aac-ahpD gene of the mini-transposon (right).