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. 2011 Sep 22;7(9):e1002263. doi: 10.1371/journal.ppat.1002263

Figure 4. Growth of M. pneumoniae in modified Hayflick medium containing different carbon sources.

Figure 4

One hundred milliliters of medium were inoculated with 5 mg of the relevant M. pneumoniae cells and incubated for up to six days at 37°C in 150-cm2 cell culture flasks. The following strains were used: wild type (wt), glpQ::Tn, mpn566::Tn, and glpD::Tn, glpQ::Tn + glpQ (complemented mutant) and glpQ::Tn + cv (control strain carrying the empty vector used for complementation). Glucose (A) and glycerol (B) were added to a final concentration of 1% (wt/vol). Attached cells were collected by scraping and growth was monitored by determination of the wet weight of the cell pellets. All measurements were done three times. Results are from a representative experiment.