Figure 5. Growth and actin organization defects in conditional mutants of proteasome components.
(A) Growth of strains carrying temperature sensitive alleles of proteasome genes were monitored and compared to the growth of a wild-type strain (BY4741) in a TECAN microplate reader at 37°C for 24 hr. (B and C) Strains carrying Ts− alleles in proteasome component genes were grown to mid-log, shifted to 37°C for 2 hr, fixed, stained with rhodamine phalloidin and visualized by fluorescence microscopy. (B) Proteasome mutant strains with actin organization defects. (C) Proteasome mutant strains that do not have actin organization defects.