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. 2011 Sep 22;7(9):e1002288. doi: 10.1371/journal.pgen.1002288

Figure 5. Growth and actin organization defects in conditional mutants of proteasome components.

Figure 5

(A) Growth of strains carrying temperature sensitive alleles of proteasome genes were monitored and compared to the growth of a wild-type strain (BY4741) in a TECAN microplate reader at 37°C for 24 hr. (B and C) Strains carrying Ts alleles in proteasome component genes were grown to mid-log, shifted to 37°C for 2 hr, fixed, stained with rhodamine phalloidin and visualized by fluorescence microscopy. (B) Proteasome mutant strains with actin organization defects. (C) Proteasome mutant strains that do not have actin organization defects.