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. 2011 Sep 22;7(9):e1002294. doi: 10.1371/journal.pgen.1002294

Figure 2. Effects of deletion of 1α(OH)ase or PTH on the skeletal development of CaR–deficient mice.

Figure 2

(A) Representative contact radiographs of femurs from 2-week-old wild-type (WT), CaR−/−, 1α(OH)ase−/−, PTH−/−, CaR−/−1α(OH)ase−/− and CaR−/−PTH−/− mice. (B) Representative longitudinal and cross sections of three-dimensional reconstructed proximal ends of tibiae and mid shaft diaphyses utilizing micro-CT. (C, E) Representative micrographs of decalcified paraffin-embedded sections of the proximal ends of tibiae stained histochemically for total collagen. (D, F) Representative micrographs from undecalcified sections of the proximal ends of tibiae stained by the von Kossa procedure. Scale bars represent 200 µm in C and E and 25 µm in D and F. (G, I) Trabecular bone volume relative to tissue volume (BV/TV, %). (H, J) Osteoid volume relative to bone volume (OV/BV, %). Each value is the mean±SEM of determinations in 6 mice of the same genotype. **, P<0.01; ***, P<0.001 compared with wild-type mice; #, P<0.05; ##, P<0.01; ###, P<0.001 compared with CaR−/− mice.