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. 2011 Sep 22;7(9):e1002294. doi: 10.1371/journal.pgen.1002294

Figure 5. Effects of deletion of 1α(OH)ase or PTH on osteoblastic bone formation in CaR–deficient mice.

Figure 5

Representative micrographs of paraffin-embedded sections of tibiae from 2-week-old wild-type (WT), CaR−/−, 1α(OH)ase−/−, PTH−/−, CaR−/−1α(OH)ase−/− and CaR−/−PTH−/− mice stained (A, C) with HE, (B, D) or immunohistochemically for osteocalcin (OCN). Scale bars represent 50 µm in A–D. (E, F) Number of osteoblasts per tissue area (N.Ob/T.Ar, #/mm2). (G, H) Osteocalcin-positive area as a percent of the tissue area. (I, J) Real-time RT–PCR of long bone tissue extracts for the expression of ALP, type I collagen (Col I) and OCN. Messenger RNA expression assessed by real-time RT–PCR is calculated as a ratio to the GAPDH mRNA level and expressed relative to levels of WT mice. Each value is the mean ± SEM of determinations in 6 animals of the same genotype. *, P<0.05; **, P<0.01; ***, P<0.001 compared with wild-type mice; #, P<0.05; ##, P<0.01; ###, P<0.001 compared with CaR−/− mice.