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. 2011 Sep 22;7(9):e1002294. doi: 10.1371/journal.pgen.1002294

Figure 6. Effects of deletion of 1α(OH)ase or PTH on osteoclastic bone resorption in CaR–deficient mice.

Figure 6

(A, B) Representative micrographs of paraffin-embedded sections of tibiae from 2-week-old wild-type (WT), CaR−/−, 1α(OH)ase−/−, PTH−/−, CaR−/−1α(OH)ase−/− and CaR−/−PTH−/− mice stained histochemically for tartrate-resistant acid phosphatase activity (TRAP). Scale bars in A and B represent 50 µm. (C, D) Number of TRAP-positive osteoclasts per tissue area (N.Oc/T.Ar, #/mm2) and (E, F) the surface of osteoclasts relative to the bone surface (Oc.S/B.S, %). Each value is the mean ± SEM of determinations in 6 animals in each group. (G, H) Real-time RT–PCR was performed on long bone tissue extracts for RANKL and OPG mRNA as described in Materials and Methods. Messenger RNA expression assessed by real-time RT–PCR analysis is calculated as a ratio to the GAPDH mRNA level and expressed relative to levels of wild-type mice. Ratio of RANKL/OPG relative mRNA levels was calculated. Each value is the mean ± SEM of determinations in 6 animals of the same genotype. *, P<0.05; **, P<0.01; ***, P<0.001 compared with wild-type mice; #, P<0.05; ##, P<0.01; ###, P<0.001 compared with CaR-/- mice.