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. 2011 Sep 22;7(9):e1002291. doi: 10.1371/journal.pgen.1002291

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Lu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panels A and B: wild-type (control) blastocysts and BCCIP deficient blastocysts were cultured in vitro. Panel A shows representative images of the cultured wild-type (control, top row) and BCCIP deficient blastocysts (bottom row) at days 0, 2, 4 and 5 in vitro (equivalent to E3.5, E5.5, E7.5 and E8.5 in vivo). ICM: inner cell mass; TG: trophoblast. Panel B illustrates the average area of inner cell mass (ICM) formed in culture by the control and BCCIP deficient blastocysts. Shown are the averages of 28 blastocysts from each group, and error bars indicate the standard errors of the averages. There is no significant difference on day 2 (p = 0.18), but all p-values are less than 1.0E-06 for days 3, 4, and 5 in culture.