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. 2011 Sep 22;7(9):e1002291. doi: 10.1371/journal.pgen.1002291

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Lu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The MEF4-LoxPshBCCIP (passage 1) cells with the conditional BCCIP knockdown cassette were infected with control retro-viruses that express YFP, and the Cre-expressing retrovirus to activate the knockdown of BCCIP. Then, the metaphase chromosome spreads (panels A and B), sister chromatid exchange assay (panels C and D), and telomere FISH (panel E and F) were produced (see Materials and Methods) to score chromosome abnormalities. Representative sister chromatid union (SCU), sister chromatid exchanges (SCE), chromatid break with single chromatid fragment (sSCF) Panel B), and chromosome fragments are shown in panels A-F. Panel G showed the frequency of three type of chromatid abnormality in 109 control and 137 BCCIP-knockdown cells. Panel H shows the frequency of sister chromatid exchanges in 41 controls and 21 BCCIP knockdown cells. Panel I illustrates the frequency of loss of telomere signals in 31 controls and 37 BCCIP knockdown cells.