Figure 4. Knockdown of PPARδ gene expression does not affect GW501516-mediated inhibition of MCP-1 expression in mouse proximal tubular (mProx) cells treated with palmitate or TNFα.

(A) Representative immunoblot showing protein expression of PPARδ in siRNA-mediated PPARδ-knockdown and control cells. (B) mRNA expression of PPARδ-target genes (PDK4 and ADRP) in PPARδ-knockdown and control cells treated with or without GW501516. Results are expressed as fold change relative to the mRNA from the control group. PDK4: Pyruvate dehydrogenase kinase 4; ADRP: Adipocyte differentiation-related protein. The effect of PPARδ knockdown on GW501516-mediated inhibition of palmitate- (C) and TNFα- (D) induced MCP-1 expression. Results are expressed as fold change relative to the mRNA from the control group. Data are shown as means ± SEM of three independent experiments. MCP-1: monocyte chemoattractant protein-1; TNFα: tumor necrotic factor α.