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. 2011 Sep 22;6(9):e25271. doi: 10.1371/journal.pone.0025271

Figure 6. GW501516 inhibits palmitate-induced NFκB activation via inhibition of TAK1 in mouse proximal tubular (mProx) cells.

Figure 6

(A) Immunoblot analysis showing palmitate-induced phosphorylation of IκB in the total cell lysate and nuclear translocation of the p65 subunit in a time course experiment. (B) Immunoblot analysis showing the effect of GW501516 on palmitate-induced phosphorylation of IκB and nuclear translocation of p65. The pretreatment time with GW501516 was 3 hours and the stimulation time with palmitate was 3 hours for phosphorylation of IκB and nuclear translocation of p65. The images are representative pictures from three independent experiments. (C) Effect of GW501516 on DNA binding of p65 to the MCP-1 promoter determined by chromatin immunoprecipitation (ChIP) analysis in palmitate-treated mProx. (D) Immunoblot analysis showing palmitate-induced phosphorylation of MAPKs (P-ERK1/2, P-p38, P-JNK) and TAK1 in a time course experiment. (E) Immunoblot analysis showing the effect of GW501516 on phosphorylation of MAPKs and TAK1. Pre-incubation time with GW501516 was 3 hours, and stimulation time with palmitate was 1 hour. Images are representative figures from three independent experiments. TAK1: TGF-β activated kinase 1. (F) Quantitative results of the ratio of band density of P-TAK1 and T-TAK1.