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. 2011 Sep 22;6(9):e25271. doi: 10.1371/journal.pone.0025271

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Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunoblot analysis showing TNFα-induced phosphorylation of IκB and TAK1 (A) and nuclear translocation of the p65 subunit (B) in a time course experiment. (C) Immunoblot analysis showing the effect of GW501516 on TNFα-induced phosphorylation of IκB and nuclear translocation of p65. The pretreatment time with GW501516 was 3 hours and the stimulation time with TNFα was 5 minutes for phosphorylation of IκB and 10 minutes for nuclear translocation of p65. Images are representative results from three independent experiments. (D) Effect of GW501516 on DNA binding of p65 to the MCP-1 promoter determined by chromatin immunoprecipitation (ChIP) analysis in TNFα-treated mProx cells. (E) Immunoblot analysis showing the effect of GW501516 on TAK1 phosphorylation. Pre-incubation time with GW501516 was 3 hours and stimulation time with TNFα was 5 minutes. (F) Quantitative result of the ratio of band density of P-TAK1 and T-TAK1. Images are representative figures from three independent experiments. TAK1: TGF-β activated kinase 1; TNFα: tumor necrotic factor α.