Figure 8. Knockdown of PPARδ gene expression does not affect the GW501516-mediated inhibitory effect on the TAK1-NFκB pathway in mouse proximal tubular (mProx) cells treated with palmitate or TNFα.

Immunoblot analysis showing the effect of GW501516 on palmitate- (A) and TNFα- (B) induced phosphorylation of IκB and TAK1 in total cell lysate and nuclear translocation of the p65 subunit in siRNA-mediated PPARδ-knockdown and control cells. Images are representative figures from three independent experiments. TAK1: TGF-β activated kinase 1; TNFα: tumor necrotic factor α.