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. 2011 Sep 22;6(9):e25271. doi: 10.1371/journal.pone.0025271

Figure 9. GW0742 inhibits palmitate- and TNFα-induced MCP-1 expression as well as TAK1 phosphorylation in mouse proximal tubular (mProx) cells.

Figure 9

mRNA expression of MCP-1 determined by real-time PCR in cultured mProx cells stimulated by 150 µM palmitate (A) or 10 nM TNFα (B) for 12 hours, with or without a 3-hour pretreatment with GW0742 (5 µM GW0742 dissolved in 0.05% DMSO). Results are expressed as fold change relative to control mRNA levels. (C) Immunoblot analysis showing palmitate-induced phosphorylation of TAK1. (D) Quantitative result of the ratio of band density of P-TAK1 and T-TAK1. (E) Immunoblot analysis showing TNFα-induced phosphorylation of TAK1. (F) Quantitative result of the ratio of band density of P-TAK1 and T-TAK1. MCP-1: monocyte chemoattractant protein-1. TNFα: tumor necrotic factor α; TAK1: TGF-β activated kinase 1. Data are shown as means ± SEM of at least three independent experiments.