Table 1. Content of cholesterol and triglycerides in lipoproteins.
Cholesterol (mg/dL) | Triglyceride (mg/dL) | ||||||||
CM | VLDL | LDL | HDL | CM | VLDL | LDL | HDL | ||
RD | Control | 0.1±0.0 | 2.9±0.2 | 11.1±0.1 | 59.4±5.8 | 0.6±0.1 | 15.6±1.4 | 9.5±1.1 | 1.7±0.2 |
Bmal1-/- | 0.3±0.0* | 7.3±0.8* | 20.5±0.3* | 35.2±4.8* | 4.6±0.5* | 19.4±2.1 | 12.7±1.4* | 2.3±0.2* | |
HFD | Control | 0.2±0.0 | 5.7±0.6 | 25.9±3.8 | 123.2±20 | 3.5±0.4 | 18.8±1.9 | 6.5±0.7 | 1.8±0.1 |
Bmal1-/- | 0.3±0.0 | 8.3±1.0* | 128.2±14* | 184.7±23* | 5.3±0.6* | 9.1±1.1* | 14.9±1.8* | 3.4±0.4* |
Blood samples were collected at ZT 10. Plasma samples were prepared from male control mice and Bmal1 -/- mice fed either a regular diet (RD) or a high fat diet (HFD) for 5 weeks. Plasma lipoproteins were separated and analyzed by an on-line dual enzymatic method for simultaneous quantification of cholesterol and triglycerides by HPLC. Data represent the means ± SEM (n = 5 for each genotype). Asterisks indicate significant differences (P<0.05).