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. 2011 Sep 22;6(9):e25289. doi: 10.1371/journal.pone.0025289

Figure 1. Recombinant HPPD protein accumulation and phenotype of transplastomic tobaccos.

Figure 1

(A) Coomassie stained SDS-PAGE and (B) 2D gels of separated protein extracts from leaves of wild type plants (Lane 1 in panel A, Gel 1 in panel B) and transplastomic tobacco plants accumulating the Pseudomonas fluorescens p-hydroxyphenyl pyruvate dioxygenase (HPPD) (Lane 2 in panel A, Gel 2 in panel B). The position of the large subunit of Rubisco (LSU) and of the recombinant HPPD is indicated. Molecular weight markers are shown on the left of panel A (Lane M) together with their molecular weight in kDa. (C) Mature wild-type (2) and transplastomic plants accumulating recombinant HPPD protein (1,3) observed 15 weeks after sowing and showing an absence of phenotypic defects.