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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Immunol. 2011 Aug 26;187(7):3694–3703. doi: 10.4049/jimmunol.1101872

FIGURE 2.

FIGURE 2

Characterization of Be-specific T cell hybridomas. A, Expression of TCR and human CD4 on the 1332-28 T cell hybridoma. Constructs encoding the variable domain of the TCR α- and β-chains of Be-specific T cell clones were transduced into the TCR− murine T cell hybridoma, 5KC. The resultant T cell hybridomas were stained with anti-human CD4 and anti-TCR mAbs and analyzed on a FACSCaliber flow cytometer. B, Stimulation of T cell hybridomas, designated 1332-28, -22, and -2, with varying BeSO4 concentrations presented by HLA-DP2-transfected murine fibroblasts. The mean ± SEM IL-2 (pg/ml) is shown, and data are representative of three independent experiments. C, Inhibition of Be-induced IL-2 secretion by the addition of various concentrations of the anti-DP mAb, B7.21, is shown.