Figure 1.
Experimental timeline and blood alcohol level cycling. (A) Pharmacological testing and time points for tissue collection for Western analysis occurred after approximately 8–10 weeks of chronic intermittent ethanol vapor exposure. (B) All analyses were conducted when ethanol-dependent animals were in acute (6hr) withdrawal, a time point coinciding with diminished blood alcohol levels (BALs) and increased ethanol self-administration behavior. Asterisks indicate ***p<0.001 significant effect of withdrawal time point.