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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Immunol. 2011 Aug 26;187(7):3888–3894. doi: 10.4049/jimmunol.1101629

Figure 3.

Figure 3

The active component in serum contains IgG. Serum from older MRL.Faslpr mice was collected and separated into IgG and non-IgG containing fractions. Unfractionated serum (black bars), IgG fraction (hatched bars), non-IgG fraction (gray bars) or PBS (open bars) was then infused into hCD20.BALB/c mice as described in Methods. Mice were then treated with 2H7 and the fraction of B cells in the indicated tissues was measured by FACS as previously reported (10) and is plotted as a percent of the average of the value of untreated control mice analyzed simultaneously. (A) PB, (B) LN, and (C) Spleen. Bars show the mean and error bars the SEM. Data combined from two independent experiments with n=7-8 mice per group. ***, p <0.0001 by Student's unpaired t test.