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. Author manuscript; available in PMC: 2012 Sep 21.
Published in final edited form as: Bioconjug Chem. 2011 Aug 25;22(9):1811–1823. doi: 10.1021/bc2002117

Figure 4. Cell surface sialic acid measured by flow cytometry.

Figure 4

BJA-B K20 cells were grown in serum-free media supplemented with 100 µM 1–6. (A) Total sialic acid detected by PAL; (B) Siaα2–6 galactose (Siaα2–6Gal) epitope detected by SNA lectin; (C) Siaα2–3Gal epitope detected by MAL-II lectin; (D) unsialylated terminal N-acetyllactosamine (LacNAc) detected by ECA lectin; (E) unsialylated terminal N-acetylgalactosamine (GalNAc) detected by HPA lectin. Error bars represent the standard deviation for three technical replicates.