Figure 5. Photocrosslinking of Siglec CD22 in BJA-B K20 and Daudi cells.
BJA-B K20 or Daudi cells were cultured with photocrosslinking sugars, photoirradiated, and lysed. Lysates from BJA-B K20 cells cultured with diazirine-containing sugars and exposed to UV light (A) or no UV light (B). Lysates from Daudi cells cultured with diazirine-containing sugars and exposed to UV light (C) or no UV light (D). For all blots, β-actin was used as a control to demonstrate similar levels of protein loading in every lane (data not shown).