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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Biotechnol Bioeng. 2011 May 10;108(11):2716–2726. doi: 10.1002/bit.23193

Figure 3.

Figure 3

Colony-forming efficiency of MSC clones as a function of trilineage potential. Clones were frozen in situ within 96-well microplates, thawed, and amplified to obtain ∼2 × 103 cells/clone (Passage 3). Potency of the expanded clones was confirmed. Colony-forming efficiency was calculated as the percentage of P3 MSC clones to form colonies when inoculated at 100 ± 10 cells in a 10-cm culture dish containing CCMA. After two weeks of cultivation, colonies were detected by Crystal Violet staining. Nomenclature: See Figure 2 legend. Median (__, n=5 clones). *, P < 0.01 vs. OAC clones.