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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Immunol. 2011 Sep 7;187(7):3663–3670. doi: 10.4049/jimmunol.1100103

Figure 1. Src kinase inhibitors prevent the T cell stop signal.

Figure 1

A) Schematic of methods used to distinguish between motile and non-motile cells. B) The fraction of migratory cells significantly decreased with OKT3 treatment, and was prevented by addition of 5 μM PP2 (representative data from over 15 experiments, 32 replicates each, mean +/- STDEV). C) Src kinase inhibitors reversed the OKT-3 mediated T cell stop signal. Cells were pre-treated with 5 μM of Src inhibitors PP1, PP2, SKI-1 and SU6656 (mean +/- STDEV, n=3, p<0.05 by ANOVA). D) SU6656 inhibits T cell adhesion to ICAM-1. Cells were incubated for 30 minutes with indicated concentrations (mean +/- STDEV, n=3, p<0.05 by ANOVA). E) PP2 and SU6656, but not SKI-1, inhibited phosphorylation of ZAP-70 and LAT. Cells were incubated for 30 minutes with compounds and stimulated for 3 minutes with OKT3 as described in methods. Representative blots from three independent experiments are shown. F) Quantification of Western Blots (mean +/- STDEV, n=3, p<0.5 ANOVA with Tukey's post-hoc test).