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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Immunol. 2011 Sep 7;187(7):3663–3670. doi: 10.4049/jimmunol.1100103

Figure 4. PP2, but not PGE2 or colchicine, impairs TCR-induced phosphorylation of ZAP-70 and LAT.

Figure 4

A) Western blot analysis of whole cell lysate. Cells were treated with 5 μM of compounds. OKT3 induced total tyrosine phosphorylation (4G10) and phosphorylation of ZAP-70 at Y319 and phosphorylation of LAT at Y191, which was prevented by treatment with PP2 but not PGE2 or colchicine. PP2 but not PGE2 or colchicine also impaired phosphorylation of Fyn. For analysis of Fyn, lysates were immunoprecipitated with Fyn antibody and blotted with p-Src-418 antibody. One representative blot from three experiments is shown. B) Quantification of Western Blots (mean +/- SEM, n=3, p<0.05 ANOVA)