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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Immunol. 2011 Aug 31;187(7):3704–3711. doi: 10.4049/jimmunol.1101823

FIGURE 3.

FIGURE 3

Phylogram and sequence alignment to the VH3-7*01 germline sequence of the heavy variable chain genes of Abs 4A10 (cyan), 2O10 (orange), 4K8/6D9 (medium blue), and 2K11 (green) from hybridoma technology and pyrosequencing. Five-letter alphanumeric labels denote sequences derived from pyrosequencing; hybridoma names are italicized. The location of the CDRs (based on IMGT analysis) is shown on top; Kabat numbering is shown at the bottom. Amino acids similar to the VH3-7*01 germline sequence (for the VH region) or to the consensus sequence (for the D/J regions) are in light gray, dissimilar amino acids in white. Variable gene segment (V-GENE) encoded sequences are separated from the diversity and joining gene segment (D/J-GENE) encoded sequences by a dashed line. Residues within the VH region with evidence of convergence are identified with an asterisk at the bottom. The phylogram was generated based on the protein sequences with MacVector software version 12 using neighbor joining, best tree, symmetric tie breaking, uncorrected (“p”) distance settings, and rooted to the deduced VH3-7*01 germ line protein sequence.