Fig. 1.

SP600125 significantly inhibits regenerative neurite outgrowth. a Representative micrographs of MDN cultures at 3 days after scratch lesion (upper panel, phase contrast; lower panel, TH-Cy2 (green) and DAPI (blue)). Cultures were treated with the pan-JNK-inhibitor SP600125 (5 μM) or DMSO control. Arrow, site of mechanical transection (scratch lesion). Scale bar, 150 μm. b Treatment with the pan-JNK-inhibitor SP600125 (5 μM) significantly impairs neurite regeneration of all primary cultured neurons including TH-positive DAergic subpopulation after mechanical transection. Data are from three independent experiments each. Bars represent means ± SEM; ***p < 0.001