Table 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| Vibrio cholerae | ||
| N16961 | O1 El Tor biotype | S. Payne |
| E7946 | O1 El Tor biotype | K. Klose |
| E7946 vc0212::kan | E7946 with kanamycin resistance cassette in vc0212 | This work |
| E7946 vc0212::kan, vc0212+ | vc0212::kan, pBADVc0212 | This work |
| E7946 vc0212::kan, lpxMYP+ | vc0212::kan, pBADLpxMYP | This work |
| E7946 vc0212::kan, lpxMEC+ | vc0212::kan, pBADLpxMEC | This work |
| C6706 | O1 El Tor biotype, lacZ- | Cameron et al. (2008) |
| C6706 Δvc0212 | C6706 containing transposon insertion in vc0212 | Cameron et al. (2008) |
| C6706 Δvc0212, vc0212+ | C6706 Δvc0212, pBADVc0212 | This work |
| O395 | O1 Classical biotype | K. Klose |
| MO10 | Wild-type O139 | K. Klose |
| Escherichia coli | ||
| JM109 | endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F′traD36, proAB, laqIqZΔM15] | Promega |
| SM10λpir | λpir recA Kanr; host and mobilizing strain for pGP704 derivatives | Miller and Mekalanos, 1988 |
| XL-1 Blue | recA1 endA1 gyrA96thi-1 hsdR17 supE44 relA1 lac[F′proAB lacIqZΔM15::Tn10 (TetR)] | Stratagene |
| W3110 | Wild type, F- l- rph-1 INV(rrnD, rrnE)1 rph-1 | E. coli Genetic Stock center (Yale) |
| MLK1067 | W3110 lpxM::Ωcam | Karow and Georgopoulos (1992) |
| Plasmids | ||
| pET21a | Vector containing a T7 promotor, Ampr | Novagen |
| pVc0212 | pET21a containing vc0212 | Hankins and Trent (2009) |
| pWSK29 | Low-copy-number cloning vector, Ampr | Wang and Kushner (1991) |
| pWVc0212 | pWSK29 containing vc0212 | This work |
| pGEM-T Easy | Vector containing a T7 promotor, Ampr | Promega |
| pGEMVc0212 | pGEM-T Easy containing vc0212 | This work |
| pGEMVc0212Kan | pGEM-T Easy containing vc0212::kan | This work |
| pUC4K | Vector containing kan resistance gene Kanr | Pharmacia |
| pHM5 | Suicide vector pGP704 carrying sacB; Ampr SucS | Runyen-Janecky et al. (1999) |
| pHM5Vc0212Kan | pHM5 carrying vc0212::kan | This work |
| pBAD18 | Arabinose-inducible expression vector, Ampr | Guzman et al. (1995) |
| pBADVc0212 | pBAD18 containing vc0212 | This work |
| pBADLpxMYP | pBAD18 containing Y. pestis lpxM (ypO2063) | This work |