Fig. 7.
Confocal laser scanning microscopy images and biomass measurements of 24 h biofilms formed on salivary pellicle-coated coverslips, comparing biofilm structures of (A) S. pyogenes MGAS6180 and (B) strain H360, and corresponding isogenic ΔaspA mutants and complemented strains ΔaspA (aspA+). Biomass data were obtained by crystal violet assay (A595) of corresponding biofilms formed on coverslips. One unit is equivalent to the grid square side length. Data are averages ± SD of three independent experiments. **P < 0.005 relative to MGAS6180 (A) or H360 (B) wild-type strains.