Stock solution volume per assay final concentration

1. 1 M Glycine NaOH buffer pH 9.2	4 μL	0.2 M
2. 100 mM DTT	0.2 μL	1 mM
3. 20 mM NAD	1 μL	1 mM
4. 50 mg/ml BSA (optional, see Note 5)	1 μL	2.5 mg/ml
5. [1,8-3H]spermidine (see Note 6)	3 μL (~150 pmol)	7.5 μM
6. 6 mg/ml eIF5A(Lys)	0.5 μL	9.0 μM
7. H2O	5.3 μL
8. Volume of master mix per tube	15 μL
9. Enzyme solution/lysate	5 μL
10. Total volume	20 μL