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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Eur J Neurosci. 2011 Sep;34(6):870–883. doi: 10.1111/j.1460-9568.2011.07802.x

Figure 8.

Figure 8

A. Live crayfish were incubated in BrdU for 24 hours and then maintained in fresh pond water for 7 days. Just before sacrifice, they were treated with EdU (green) for 6 hours. Fixed brains also were labeled immunocytochemically for glutamine synthetase (blue) to reveal the niche and streams. S phase cells in the niche and streams are labeled only with EdU, demonstrating that migration is uni-directional (away from the niche) and that niche cell divisions are not self-renewing, as no BrdU-labeled cells remain in the niche. B. Higher magnification image of the LPZ. The BrdU-labeled cells which were labeled first in the sequence are found only in the proliferation zones (MPZ and LPZ) and not in the niche. Arrow indicates direction of migration. Scale bars: A, 100 µm; B, 50 µm. (Images from Benton et al., 2011).