Skip to main content
. Author manuscript; available in PMC: 2011 Sep 23.
Published in final edited form as: Methods Mol Biol. 2011;720:207–216. doi: 10.1007/978-1-61779-034-8_13

Fig. 2.

Fig. 2

Ion exchange chromatographic separation of deoxyhypusine and hypusine. The proteins in the DOHH reaction mixture were precipitated with TCA and hydrolyzed in 6 N HCl. The amino acids in the hydrolyzed proteins were separated by ion exchange chromatography on DC6A column using PA Buffer B. Hypusine is eluted from the column 2 min earlier than deoxyhypusine.