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| 1 M Tris–HCl pH 7.5 | 0.8 μl | 20 mM |
| 100 mM DTT | 2.4 μl | 6 mM |
| 50 mg/ml BSA | 0.8 μl | 1 mg/ml |
| eIF5A([3H]-Dhp) (2 μM) | 2 μl | 0.1 μM |
| H2O | 14 μl | |
| Volume of master mix per tube | 20 μl | |
| Enzyme solution/lysate (see Note 6) | 20 μl | |
| Total volume | 40 μl |
