Table 1.
Electromagnetic Wave-Assisted Proteolysis Approaches
| Proteolysis approaches | Electromagnetic wave | Digestion medium | Protease | Digestion time | Ref. |
|---|---|---|---|---|---|
| In-solution proteolysis | Microwave | Water | Trypsin | 10 min | [20] |
| In-solution proteolysis | Microwave | Water | Endoproteases lysine-C | 10 min | [20] |
| In-solution proteolysis | Microwave | Organic solvent/water | Trypsin | 10 min | [21] |
| In-solution proteolysis | Microwave | Water | Pronase | 10 min | [22] |
| In-solution proteolysis | Microwave | Water | Pronase | 3 min | [23] |
| In-solution proteolysis | Microwave | Water | α-Chymotrypsin | 10 min | [22] |
| In-solution proteolysis | Microwave | Water | α-Chymotrypsin | 3 min | [23] |
| In-solution proteolysis | Microwave | Water | Pepsin | 3 min | [23] |
| In-solution proteolysis | Microwave | Water | Trypsin | 1 min | [24] |
| In-gel proteolysis | Microwave | Gel/water | Trypsin | 5 min | [24] |
| In-solution proteolysis | Microwave | Tiles/Water | Trypsin | 30 min | [25] |
| In-solution proteolysis | Microwave | Water | Trypsin | 30 min | [26] |
| In-gel proteolysis | Microwave | Gel/water | Trypsin | 5 min | [28] |
| In-gel proteolysis | Microwave | Gel/water | Trypsin | 15 min | [20] |
| In-tip proteolysis | Microwave | Monolith/water | Immobilized trypsin | 2 min | [29] |
| In-solution proteolysis | Microwave | Titania particles/water | Trypsin | 45 s | [30] |
| In-solution proteolysis | Microwave | Magnetic particles/water | Trypsin | 1 min | [16] |
| Proteolysis on magnetic particles | Microwave | Magnetic particles/water | Immobilized trypsin | 15 s | [17] |
| Proteolysis on magnetic particles | Microwave | Magnetic particles/water | Immobilized trypsin | 15 s | [31] |
| Proteolysis on magnetic particles | Microwave | Magnetic particles/water | Immobilized trypsin | 15 s | [32] |
| Proteolysis on magnetic particles | Microwave | Magnetic particles/water | Immobilized trypsin | 45 s | [33] |
| In-solution proteolysis | IR radiation | Water | Trypsin | 5 min | [37] |
| In-solution proteolysis | IR radiation | Water | α-Chymotrypsin | 5 min | [40] |
| In-gel proteolysis | IR radiation | Gel/water | Trypsin | 5 min | [41] |
| On-plate proteolysis | IR radiation | Water | Trypsin | 5 min | [42] |
| In-chip proteolysis | IR radiation | Water | Trypsin | 6 min | [43] |
| On-microsphere proteolysis | IR radiation | Water | Immobilized trypsin | 5 min | [44] |
| On-plate proteolysis | UV radiation | Water | Trypsin | 5 min | [42] |
| In-chip proteolysis | Laser radiation | Water | Trypsin | 30 s | [45] |
| In-gel proteolysis | Laser radiation | Gel/water | Trypsin | 45 s | [45] |
| On-plate proteolysis | Laser radiation | Water | Trypsin | 5 s | [45] |