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. 2011 Sep 12;108(38):16002–16007. doi: 10.1073/pnas.1109493108

Fig. 5.

Fig. 5.

S100A4+ stromal cells provide VEGF-A to the metastatic microenvironment to support metastatic colonization. (A) Representative images and quantification of VEGF-A (green) and S100A4-tk (red) immunostaining in metastatic lung tissue of control and GCV-treated S100A4-tk mice. (Scale bars: 20 μm.) (B) Primary tumor volume of S100A4-Cre;VEGF-Aflox/flox mice and control littermates 24 d after 4T1 orthotopic implantation. Representative H&E-stained lung sections and quantification of metastatic area from S100A4-Cre;VEGF-Aflox/flox (S100A4-Cre;VEGF-Af/f) mice and control littermates after 4T1 orthotopic (C) and 4T1 i.v. (D) injections (n = 5–8 in each group). (Scale bars: C, 50 μm; D, 100 μm). (E) Microvessel density was quantified by CD31 staining (red) with DAPI nuclear counterstain (blue) within metastatic lesions of these mice. (Scale bars: 50 μm.) Dotted lines encircle metastatic lesions. Mean ± SEM. **P < 0.01; ***P < 0.001.